Gradient

Senior Scientists

Robyn Prueitt, Ph.D., DABT
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Education

Ph.D., Cell and Molecular Biology, University of Texas Southwestern Medical Center at Dallas

B.S., Biology, Pacific Lutheran University

Expertise

  • Molecular Biology & Toxicology
  • Carcinogenesis
  • Mode-of-action Analyses
  • Human Genetics
  • Molecular Epidemiology

Robyn Prueitt, Ph.D., DABT

Senior Toxicologist

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Dr. Prueitt is an expert in human genetics, carcinogenesis, and molecular toxicology. At Gradient, her primary responsibilities include evaluating toxicology and epidemiology data for regulatory comment, human health risk assessment, and litigation projects.

Before joining Gradient, Dr. Prueitt worked as a research scientist at the Fred Hutchinson Cancer Research Center, where she designed and interpreted multi-species proteomic studies related to tumor biology and biomarker discovery. As a postdoctoral fellow at the National Cancer Institute's Laboratory of Human Carcinogenesis, Dr. Prueitt performed genomic and molecular epidemiologic studies of human cancer risk and progression.  As a pre-doctoral student, she investigated genetic causes of human diseases using various molecular genetic techniques.  Dr. Prueitt has authored several peer-reviewed articles in the fields of genetics, carcinogenesis, and toxicology. 

Education

Ph.D., Cell and Molecular Biology, University of Texas Southwestern Medical Center at Dallas

B.S., Biology, Pacific Lutheran University

Expertise

  • Molecular Biology & Toxicology
  • Carcinogenesis
  • Mode-of-action Analyses
  • Human Genetics
  • Molecular Epidemiology

Services

Representative Projects

Cytotoxicity Evaluation:  Evaluated the potential cytotoxicity of a medical device by critically reviewing the experimental data and human clinical studies for the device and its components.

Carcinogenicity Assessment:  Evaluated whether the weight of epidemiology, animal toxicity, mechanistic, and pharmacokinetic evidence indicates that toluene diisocyanate is a human carcinogen. This analysis used Gradient’s hypothesis-based weight-of-evidence approach and was published in a peer-reviewed journal.

Review of Toxicogenomics:  Critically reviewed global gene expression profiling data for a population exposed to benzene and determined whether the expression profile could be used as a biomarker of benzene toxicity in a broader population, particularly without proof of benzene exposure from a specific source.

Risk Evaluation:  Evaluated the potential lung cancer risk from exposure to asbestos during vehicle brake repair and considered the association between cigarette smoking and lung cancer in comparison to that expected from asbestos exposure.

Weight-of-Evidence Analysis:  Used Gradient’s hypothesis-based weight-of-evidence approach to assess whether the epidemiology, toxicology, and mechanistic evidence supports chlorpyrifos being a neurobehavioral toxicant in humans at relatively low exposure levels. This work was published in a peer-reviewed journal.

Bioavailability Assessment:  Assessed whether animal, mechanistic, and epidemiological data are consistent with the nickel ion bioavailability model, which asserts that the carcinogenicity of nickel-containing substances is based on the bioavailability of the nickel ion at nuclear sites of target respiratory epithelial cells.  This analysis was published in a peer-reviewed journal.

Toxicity Summary:  Classified, summarized, and entered relevant studies of lead and bisphenol A into IUCLID (International Uniform Chemical Information Database) 5.2, a database for the intrinsic and hazard properties of chemical substances that companies can use to submit data under the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) legislation in Europe.

Regulatory Comment:  Provided written and oral comments on several occasions to US EPA on clinical and epidemiology studies and their bearing on US EPA&#39s National Ambient Air Quality Standards (NAAQS) for ozone.

Selected Publications

Prueitt, RL; Rhomberg, LR; Goodman, JE.  2013. "Hypothesis-based weight-of-evidence evaluation of the human carcinogenicity of toluene diisocyanate." Crit. Rev. Toxicol. 43 : 391-435.

Goodman, JE; Prueitt, RL; Thakali, S; Oller, AR.  2011. "The nickel ion bioavailability model of the carcinogenic potential of nickel-containing substances in the lung." Crit. Rev. Toxicol. 41 : 142-174.

Prueitt, RL; Goodman, JE; Bailey, LA; Rhomberg, LR.  2011. "Hypothesis-based weight-of-evidence evaluation of the neurodevelopmental effects of chlorpyrifos." Crit. Rev. Toxicol. 41 : 822-903.

Goodman, JE; Kerper, LE; Petito Boyce, C; Prueitt, RL; Rhomberg, LR.  2010. "Weight-of-evidence analysis of human exposures to dioxins and dioxin-like compounds and associations with thyroid hormone levels during early development." Regul. Toxicol. Pharmacol. 58 : 79-99.

Prueitt, RL; Goodman, JE; Valberg, PA.  2009. "Radionuclides in cigarettes may lead to carcinogenesis via p16INK4a inactivation." J. Environ. Radioact. 100 : 157-161.